Genotyping did not evidence any contribution of Mycobacterium bovis to human tuberculosis in Brazil

Submitted by Sandra Infurna ([email protected]) on 2016-10-11T15:30:42Z No. of bitstreams: 1 alexandre_santos_etal_IOC_2011.pdf: 239001 bytes, checksum: 50d5454a5f61743435cbeee1d8103caf (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2016-10-11T15:46:25Z (GMT) No. of bitstreams: 1 alexandre_santos_etal_IOC_2011.pdf: 239001 bytes, checksum: 50d5454a5f61743435cbeee1d8103caf (MD5)Made available in DSpace on 2016-10-11T15:46:25Z (GMT). No. of bitstreams: 1 alexandre_santos_etal_IOC_2011.pdf: 239001 bytes, checksum: 50d5454a5f61743435cbeee1d8103caf (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Doenças Torácicas. Laboratório de Micobacteriologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Doenças Torácicas. Laboratório de Micobacteriologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Fundação Oswaldo Cruz. Centro de Referência Nacional em Tuberculose Prof. Hélio Fraga. Rio de Janeiro, RJ, Brasil.Clínica de Tuberculose Augusto Guimarães, Centro de Referencia Regional em Tuberculose. Program de Controle de Tuberculose. Campos, RJ, Brasil;.EMBRAPA. Divisão de Saúde Animal. Juiz de Fora, MG, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Doenças Torácicas. Laboratório de Micobacteriologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Departamento de Medicina Interna. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Pesquisa Multidisciplinar. Rio de Janeiro, RJ, Brasil.The contribution of Mycobacterium bovis to the global burden of tuberculosis (TB) in man is likely to be underestimated due to its dysgonic growth characteristics and because of the absence of pyruvate in most used media is disadvantageous for its primary isolation. In Brazil Mycobacterium culture, identification and susceptibility tests are performed only in TB reference centers, usually for selected cases. Moreover, solid, egg-based, glycerol-containing (without pyruvate supplementation) Löwenstein-Jensen (L-J) or Ogawa media are routinely used, unfavouring M. bovis isolation. To determine the importance of M. bovis as a public health threat in Brazil we investigated 3046 suspected TB patients inoculating their clinical samples onto routine L-J and L-J pyruvate enriched media. A total of 1796 specimens were culture positive for Mycobacterium spp. and 702 TB cases were confirmed. Surprisingly we did not detect one single case of M. bovis in the resulting collection of 1674 isolates recovered from M. bovis favourable medium analyzed by conventional and molecular speciation methods. Also, bacillary DNA present on 454 sputum smears from 223 TB patients were OxyR genotyped and none was recognized as M. bovis. Our data indicate that M. bovis importance on the burden of human TB in Brazil is marginal

Elastin-like hydrogel stimulates angiogenesis in a severe model of critical limb ischemia (CLI): An insight into the glyco-host response

Producción CientíficaCritical limb ischemia (CLI) is characterized by the impairment of microcirculation, necrosis and inflammation of the muscular tissue. Although the role of glycans in mediating inflammation has been reported, changes in the glycosylation following muscle ischemia remains poorly understood. Here, a murine CLI model was used to show the increase of high mannose, α-(2, 6)-sialic acid and the decrease of hybrid and bisected N-glycans as glycosylation associated with the ischemic environment. Using this model, the efficacy of an elastin-like recombinamers (ELR) hydrogel was assessed. The hydrogel modulates key angiogenic signaling pathways, resulting in capillary formation, and ECM remodeling. Arterioles formation, reduction of fibrosis and anti-inflammatory macrophage polarization wa also induced by the hydrogel administration. Modulation of glycosylation was observed, suggesting, in particular, a role for mannosylation and sialylation in the mediation of tissue repair. Our study elucidates the angiogenic potential of the ELR hydrogel for CLI applications and identifies glycosylation alterations as potential new therapeutic targets.This work was funded by Science Foundation Ireland (SFI) and the European Regional Development Fund (Grant Number 13/RC/2073) AngioMatTrain Seventh Framework Programme Grant Agreement no.: 317304.Deutsche Forschungsgemeinschaft (INST 2388/64-1, INST 2388/30-1)Ministry of Science, Research and Arts of Baden Württemberg (Az.: SI-BW 01222-91, 33-729.55-3/214)Research in the mass spectrometry laboratory at the University of Goteborg was supported by EMBO short-term fellowships, in collaboration with the Swedish infrastructure for biological mass spectrometry (BioMS) supported by the Swedish Research Council.[ASTF- 7602-2018